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pcag ert2creert2 backbone  (Addgene inc)


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  • 93

    Structured Review

    Addgene inc pcag ert2creert2 backbone
    Pcag Ert2creert2 Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcag+ert2creert2+backbone/pCAG-ERT2CreERT2+(Plasmid+%2313777)/bio_rxiv__2024__11__20__624546-146-1-7
    Average 93 stars, based on 89 article reviews
    pcag ert2creert2 backbone - by Bioz Stars, 2026-09
    93/100 stars

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    Plasmid Preparation:

    Article Title: A sequential event-responsive fluorescent reporter for inducible Cre and Flp combinatorial recombination
    Article Snippet: The purified FlpO fragment was digested with EcoRI and XmaI (Neb, EcoRI #R0101, XmaI #R0180), while the purified DD fragment was digested with XmaI and NotI (Neb, NotI-HF #R3189). .. The pCAG-ERT2CreERT2 backbone (gift from Connie Cepko; Addgene plasmid #13777; http://n2t.net/addgene:13777 ; RRID: Addgene_13777) was digested with EcoRI and NotI. .. The purified digested fragments were ligated using the T4 DNA ligase (Neb #M0202) in a ratio 1:3:3 (pCAG:FlpO:DD, w/w/w) following the manufacturer’s protocol.



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    93
    Addgene inc pcag ert2creert2 backbone
    Pcag Ert2creert2 Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcag+ert2creert2+backbone/pCAG-ERT2CreERT2+(Plasmid+%2313777)/bio_rxiv__2024__11__20__624546-146-1-7
    Average 93 stars, based on 1 article reviews
    pcag ert2creert2 backbone - by Bioz Stars, 2026-09
    93/100 stars
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